Running spaceranger as cluster mode that uses Sun Grid Engine (SGE) as queuing.
There are 2 steps to analyze Spatial RNA-seq data.
Step 1: spaceranger mkfastq
demultiplexes raw base call (BCL
) files generated by Illumina sequencers into FASTQ files.
Step 2: spaceranger count
takes FASTQ files from spaceranger mkfastq
and performs alignment, filtering, barcode counting, and UMI counting.