{"id":2026,"date":"2022-06-08T11:39:43","date_gmt":"2022-06-08T15:39:43","guid":{"rendered":"http:\/\/sys4seq.com\/?page_id=2026"},"modified":"2022-06-08T11:39:43","modified_gmt":"2022-06-08T15:39:43","slug":"proteomics","status":"publish","type":"page","link":"https:\/\/sys4seq.com\/index.php\/proteomics\/","title":{"rendered":"Proteomics"},"content":{"rendered":"<div id=\"cherry-posts-list-1\" class=\"cherry-posts-list template-default  \"><div class=\"cherry-posts-item post-item item-0 odd\"><div class=\"inner cherry-clearfix\"><figure class=\"post-thumbnail\"><\/figure>\n<h4 class=\"post-title\"><a href=\"https:\/\/sys4seq.com\/index.php\/2020\/04\/04\/quantitative-proteomics-tmt-based-quantitation-of-proteins\/\" title=\"Quantitative proteomics : TMT-based quantitation of proteins\">Quantitative proteomics : TMT-based quantitation of proteins<\/a><\/h4>\n<div class=\"post-meta\">\n\tPosted on <time datetime=\"2020-04-04T11:21:06-04:00\">April 4, 2020<\/time> by <span class=\"post-author vcard\"><a href=\"https:\/\/sys4seq.com\/index.php\/author\/adinasarapu\/\" rel=\"author\">Ashok Dinasarapu<\/a><\/span>  <span class=\"post-tax post-tax-category\"><a href=\"https:\/\/sys4seq.com\/index.php\/category\/analysis\/\">Analysis<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/category\/omics\/\">Omics<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/category\/proteomics\/\">Proteomics<\/a><\/span>\n<\/div>\n\n<div class=\"post-content part\">Quantification of proteins using isobaric labeling (tandem mass tag or TMT) starts with the reduction of disulfide bonds in proteins with Dithiothreitol (DTT).\u00a0Alkylation with iodoacetamide (IAA) after cystine reduction results in the covalent addition of a carbamidomethyl group that prevents the formation of disulfide bonds. Then, overnight digestion of the proteins using trypsin or trypsin\/LyC<\/div>\n<a href=\"https:\/\/sys4seq.com\/index.php\/2020\/04\/04\/quantitative-proteomics-tmt-based-quantitation-of-proteins\/\" class=\"btn btn-default\">read more<\/a>\n<footer><span class=\"post-tax post-tax-post_tag\"><a href=\"https:\/\/sys4seq.com\/index.php\/tag\/omics\/\">Omics<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/tag\/proteomics\/\">Proteomics<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/tag\/tmt\/\">TMT<\/a><\/span><\/footer><\/div><\/div><!--\/.cherry-posts-item--><div class=\"cherry-posts-item post-item item-1 even\"><div class=\"inner cherry-clearfix\"><figure class=\"post-thumbnail\"><\/figure>\n<h4 class=\"post-title\"><a href=\"https:\/\/sys4seq.com\/index.php\/2020\/04\/04\/quantitative-proteomics-label-free-quantitation-of-proteins\/\" title=\"Quantitative proteomics: label-free quantitation of proteins\">Quantitative proteomics: label-free quantitation of proteins<\/a><\/h4>\n<div class=\"post-meta\">\n\tPosted on <time datetime=\"2020-04-04T11:12:19-04:00\">April 4, 2020<\/time> by <span class=\"post-author vcard\"><a href=\"https:\/\/sys4seq.com\/index.php\/author\/adinasarapu\/\" rel=\"author\">Ashok Dinasarapu<\/a><\/span>  <span class=\"post-tax post-tax-category\"><a href=\"https:\/\/sys4seq.com\/index.php\/category\/analysis\/\">Analysis<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/category\/omics\/\">Omics<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/category\/proteomics\/\">Proteomics<\/a><\/span>\n<\/div>\n\n<div class=\"post-content part\">Liquid chromatography (LC) coupled with mass spectrometry (MS) has been widely used for protein expression quantification. Protein quantification by tandem-MS (MS\/MS) uses integrated peak intensity from the parent-ion mass (MS1) or features from fragment-ions (MS2).\u00a0Label-free quantification (LFQ) may be based on precursor ion intensity (peak areas or peak heights) or on spectral counting. Here, the<\/div>\n<a href=\"https:\/\/sys4seq.com\/index.php\/2020\/04\/04\/quantitative-proteomics-label-free-quantitation-of-proteins\/\" class=\"btn btn-default\">read more<\/a>\n<footer><span class=\"post-tax post-tax-post_tag\"><a href=\"https:\/\/sys4seq.com\/index.php\/tag\/lfq\/\">LFQ<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/tag\/omics\/\">Omics<\/a> <a href=\"https:\/\/sys4seq.com\/index.php\/tag\/proteomics\/\">Proteomics<\/a><\/span><\/footer><\/div><\/div><!--\/.cherry-posts-item--><\/div><!--\/.cherry-posts-list-->\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"_links":{"self":[{"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/pages\/2026"}],"collection":[{"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/comments?post=2026"}],"version-history":[{"count":1,"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/pages\/2026\/revisions"}],"predecessor-version":[{"id":2028,"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/pages\/2026\/revisions\/2028"}],"wp:attachment":[{"href":"https:\/\/sys4seq.com\/index.php\/wp-json\/wp\/v2\/media?parent=2026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}